20th ADNAT Convention

International Symposium on
‘Genome Editing Technologies and their Applications in Biology, Medicine and Agriculture’

16th to 18th February 2017
Venue: Convention Centre, KIIT University, Bhubaneswar

 
 

Hands-on Training cum Workshop on
CRISPR/Cas9- a robust tool for genome editing


About the workshop

“CRISPR” (pronounced “crisper”) stands for Clustered Regularly Interspaced Short Palindromic Repeats, which are the hallmark of a bacterial defense system that forms the basis for the popular CRISPR-Cas9 genome editing technology. CRISPR-Cas9 system can be programmed to target specific stretches of genetic code and to edit DNA at precise locations. CRISPR/Cas9 can be used to generate knock-out cells or animals by co-expressing a gRNA specific to the gene to be targeted and the endonuclease Cas9. These tools allow researchers to permanently modify genes in living cells and organisms and, in the future, may make it possible to correct mutations at precise locations in the human genome to treat genetic causes of disease.

This 1-week workshop will consist of a hands-on laboratory experience demonstrating targeted mutagenesis in cultured mammalian cells. A bioinformatics practical session will introduce tools for guide DNA design and the analysis of multiplexed genetic screens. Workshop faculty will address topics in genome editing and CRISPR-Cas9 research, including basic and enhanced CRISPR methods, cellular repair mechanisms, regulation of gene expression, bioinformatics, applications to various organisms, and bioethics.

Schedule:

Day 1: Basic theory (cloning, transfection & overview of CRISPR strategy),
Practical: Start gDNA cloning.

Day 2: Theory: Detailed CRISPR protocol discussion for gDNA design;
Practical: Transfection of preexisting gDNA construct, continue gDNA cloning

Day 3: Theory: Discussion on downstream steps,
Practical: Continue cloning, observe transfected cells.

Day 4: Theory: Live projects of gDNA construct design, discussion on downstream steps;
Practical: Continue cloning, observe transfected cells.

Day 5: Practical: Harvest the cells and gDNA preparation etc., [preparation for confirmation of deletion, continue cloning

Day 6: Practical: Confirmation of deletion, Complete cloning, Result analysis 

Day 7: Result analysis and Conclude workshop, Valedictory function

In addition, you will receive a detailed course guide containing all lecture materials, laboratory protocols, troubleshooting tips and more to help you

Date: 19 February- 25 February, 2017

Deadline: Last date for application is 31 January, 2017.

Eligibility: Graduate students, PhD scholars, researchers, scientists from academia and industry can participate in the workshop. Participants are expected to attend 20th ADNAT convention.
Maximum no. of participants is restricted to 20 and allocation will be on first come first serve basis. Participation will be confirmed only upon receipt of payment.

Fees: Participants with accommodation- Rs. 20,000/-, Participants without accommodation Rs. 15,000/-
Note:

  1. Accommodation will be provided in twin sharing basis in AC rooms including breakfast and dinner.
  2. All participants will be provided with lunch and tea/coffee/snacks at the workshop venue.

Payment details: Please refer to registration page.

Cancellation & refund policy: Cancellation will be allowed up to 7 days prior to commencement of ADNAT convention (09 February, 2017) and 50% fees will be refunded. No refund will be possible if cancelled at any later date.

Instructors:    Dr. Rohan Kamat, ViraVecs Labs, Bengaluru, India
Dr. Rahul Modak, KIIT University, Bhubaneswar, India

Contact: Dr. Rahul Modak
KIIT university, Bhubaneswar

Email: rahul.modak@kiitbiotech.ac.in

Download Application Form